DNA Reduction of Waterborne E.coli by Underwater Capillary Discharge

Abstract

Escherichia coli (E. coli), (190CRC) is one of the most common waterborne
pathogens. It exists in drinking water and most of the water diseases are associated with it.
The permanent inactivation of E.coli from water requires its DNA distortion, that makes it
ineffective for regeneration. The underwater plasma discharge in capillary tube induces
reactive oxidant species {OH. radicals, Ozone (O3), hydrogen peroxide (H2O2) and reactive
oxygen (O)}. Ultraviolet (UV) radiations are most important shock waves that play a vital
role for complete degradation of E.coli from water. In the current study, the effects of
plasma for different oxygen injection rates and applied voltages on DNA distortion of
E.coli have been reported. The bactericidal tests including DNA and protein leakage of
plasma-treated water showed complete distortion of E.coli DNA structure and sterilization
of water from bacteria.

Keywords: Capillary discharge, E.coli, DNA, Protein leakage, Sterilization