Investigation of Electrochemically Modulated Fluorescent Cresyl Violet Molecules for Biosensing Application Using an Electrochemical Surface Plasmon Resonance

Abstract

In this work, the potential-modulated fluorescent of cresyl violet molecules was investigated under an applied electric step potential using the electrochemical surface plasmon resonance (EC-SPR) technique. The EC-SPR device employed in the study consisted of two detection units: a reflected optical intensity detection unit and a fluorescence detection unit. Both units were used simultaneously to optimize the fluorescence signal and achieve the highest fluorescence intensity. The results show that the fluorescence intensity of the cresyl violet molecules changes during the step potential. This study demonstrates that cresyl violet molecules are suitable candidates for biosensing applications using the EC-SPR technique based on the detection of fluorescence differences between their reduced and oxidized states. This research opens up a new avenue to using this class of dyes in electrochemically modulated SPR fluorescence-based biosensors.